| overnight | 5 | temporal |
| with primary and secondary antibodies | 5 | other |
| for 30 min at 55c | 4 | temporal |
| protein → be → incubated | 24 |
| protein → be followed by → incubation | 20 |
| protein → be incubated in → absence | 3 |
| negative | neutral | positive |
| 0.050 | 0.899 | 0.051 |
| Raw frequency | 47 |
| Normalized frequency | 0.431 |
| Modifier score | 0.500 |
| Perplexity | 70.998 |