| in blocking buffer | 19 | location |
| subsequently | 11 | temporal |
| after washing three times | 11 | temporal |
| membrane → be → incubated | 144 |
| membrane → be subjected to → incubation | 3 |
| membrane → be incubated after → transfer | 3 |
| negative | neutral | positive |
| 0.048 | 0.900 | 0.051 |
| Raw frequency | 150 |
| Normalized frequency | 0.741 |
| Modifier score | 0.500 |
| Perplexity | 88.970 |