| manually | 4 | manner |
| by amplifying pcr fragments | 3 | manner |
| least twice | 2 | temporal |
| mutation → be → verified | 14 |
| mutation → be → checked | 6 |
| negative | neutral | positive |
| 0.139 | 0.806 | 0.055 |
| Raw frequency | 20 |
| Normalized frequency | 0.384 |
| Modifier score | 0.988 |
| Perplexity | 471.376 |